Korean Journal of Mycology (Kor J Mycol) 2023 June, Volume 51, Issue 2, pages 133. https://doi.org/10.4489/KJM.20230014
Received on June 02, 2023, Revised on June 26, 2023, Accepted on June 27, 2023.
Copyright © The Korean Society of Mycology.
This is an Open Access article which is freely available under the Creative Commons Attribution-NonCommercial 4.0 International License (CC BY-NC) (https://creativecommons.org/licenses/by-nc/4.0/).
INTRODUCTION
Airborne environments harbor a diverse array of biological aerosols, including bacteria, fungi, pollen, and tissues [1]. Fungal spores, particularly in outdoor and indoor air, constitute a significant focus of research in the field of aerobiology [2]. Previous studies focus on the sources and dispersal patterns of airborne fungi, temporal variations in fungal species concentrations, and their relationships with other sources. The composition of fungal communities in the air varies considerably across continents and oceans [3]. In Korea, a nationwide study identified Cladosporium as the most abundant airborne fungus, followed by Alternaria and Irpex [4].
Penicillium fungi are ubiquitous, with species found in diverse environments, including indoor and outdoor air, soil, marine habitats, and foods [5]. Among the 483 currently documented species, Penicillium is a broad genus classified into 2 subgenera and 32 sections by Houbraken et al. [6]. The systematics of Penicillium species were determined through the analysis of morphological characteristics, such as colony features, and the number and shape of conidiophore branches. Molecular phylogeny relied on DNA regions, primarily the internal transcribed spacer (ITS), β-tubulin (BenA), calmodulin (CaM), and RNA polymerase II second-largest subunit (RPB2) [7].
In Korea, numerous species of Penicillium have been documented over the years, and a comprehensive list was compiled by Kim et al. [8]. Recently, 15 previously unrecorded species were identified [912]. However, the number of reported Penicillium species in Korea remains lower than the global count. Considering the industrial significance of Penicillium species, particularly in antibiotic and food production, as well as their potential pathogenicity to crops and mycotoxin production [13], ongoing studies on Penicillium species in Korea are crucial.
In this study, we report the occurrence of P. mexicanum, which has not previously been reported in Korea. We isolated this strain from air samples and conducted morphological identification in conjunction with phylogenetic analyses utilizing the ITS, BenA, and CaM regions.
MATERIAL AND METHODS
RESULTS AND DISCUSSION
REFERENCE
Després V, Huffman J, Burrows S, Hoose C, Safatov A, Buryak G, Fröhlich-Nowoisky J, Elbert W, Andreae M, Pöschl U. Primary biological aerosol particles in the atmosphere: a review. Tellus B: Chem Physic Meteorol 2012;64:15598.
https://doi.org/10.3402/tellusb.v64i0.15598
Burge H. An update on pollen and fungal spore aerobiology. J Allerg Clinic Immunol 2002;110:544-52.
https://doi.org/10.1067/mai.2002.128674
Fröhlich-Nowoisky J, Burrows S, Xie Z, Engling G, Solomon P, Fraser M, Mayol-Bracero O, Artaxo P, Begerow D, Conrad R. Biogeography in the air: fungal diversity over land and oceans. Biogeosciences 2012;9:1125-36.
https://doi.org/10.5194/bg-9-1125-2012
Yoon W, Lim J, Kim D, Kim S, Lee HS, Kim CB, Kang JW, Lee KH, Oh I, Kim Y. Evaluation of distribution and inflammatory effects of airborne fungus in Korea. J Environ Health Sci 2019;45:638-45.
Yadav AN, Verma P, Kumar V, Sangwan P, Mishra S, Panjiar N, Gupta VK, Saxena AK. Biodiversity of the genus Penicillium in different habitats. In New and future developments in microbial biotechnology and bioengineering. Amsterdam: Elsevier; 2018. p. 3-18.
https://doi.org/10.1016/B978-0-444-63501-3.00001-6
Houbraken J, Kocsubé S, Visagie CM, Yilmaz N, Wang X-C, Meijer M, Kraak B, Hubka V, Bensch K, Samson R. Classification of Aspergillus, Penicillium, Talaromyces and related genera (Eurotiales): an overview of families, genera, subgenera, sections, series and species. Sutd Mycol 2020;95:5-169.
https://doi.org/10.1016/j.simyco.2020.05.002
Visagie C, Houbraken J, Frisvad JC, Hong SB, Klaassen C, Perrone G, Seifert K, Varga J, Yaguchi T, Samson R. Identification and nomenclature of the genus Penicillium. Stud Mycol 2014;78:343-71.
https://doi.org/10.1016/j.simyco.2014.09.001
Kim HJ, Kim JS, Cheon KH, Kim DH, Seok SJ, Hong SB. Species list of Aspergillus, Penicillium and Talaromyces in Korea, Based on'one fungus one name'system. Kor J Mycol 2016;44:207-19.
https://doi.org/10.4489/KJM.2016.44.4.207
Kim SH, Kim JS, Lim YW, Park MS. Three unrecorded Penicillium species from marine environments in south Korea. Kor J Mycol 2021;49:351-62.
Nguyen TT, Kwan Noh KJ, Lee HB. New species and eight undescribed species belonging to the families Aspergillaceae and Trichocomaceae in Korea. Mycobiology 2021;49:534-50.
https://doi.org/10.1080/12298093.2021.1997461
Pangging M, Nguyen TT, Lee HB. Seven new records of Penicillium species belonging to section Lanata-Divaricata in Korea. Mycobiology 2021;49:363-75.
https://doi.org/10.1080/12298093.2021.1952814
Kim YE, Noh HJ, Jung IH, Seo HJ, Chi SY, Kim SH. Five undescribed fungal species isolated from indoor air of preservation rooms of unsterilized records in the national archives of Korea. J Odor Ind Environ 2022;21:207-14.
https://doi.org/10.15250/joie.2022.21.3.207
Kim YE, Noh HJ, Jung IH, Seo HJ, Chi SY, Kim SH. Five undescribed fungal species isolated from indoor air of preservation rooms of unsterilized records in the national archives of Korea. J Odor Ind Environ 2022;21:207-14.
https://doi.org/10.15250/joie.2022.21.3.207
White TJ, Bruns T, Lee S, Taylor J. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. PCR protocols: a guide to methods and applications 1990;18:315-22.
https://doi.org/10.1016/B978-0-12-372180-8.50042-1
Gardes M, Bruns TD. ITS primers with enhanced specificity for basidiomycetes-application to the identification of mycorrhizae and rusts. Mol Ecol 1993;2:113-8.
https://doi.org/10.1111/j.1365-294X.1993.tb00005.x
Glass NL, Donaldson GC. Development of primer sets designed for use with the PCR to amplify conserved genes from filamentous ascomycetes. Appl Environ Microbiol 1995;61:1323-30.
https://doi.org/10.1128/aem.61.4.1323-1330.1995
Hong SB, Cho HS, Shin HD, Frisvad JC, Samson RA. Novel Neosartorya species isolated from soil in Korea. Int J Sys Evol Microbiol 2006;56:477-86.
https://doi.org/10.1099/ijs.0.63980-0
Hong SB, Cho HS, Shin HD, Frisvad JC, Samson RA. Novel Neosartorya species isolated from soil in Korea. Int J Sys Evol Microbiol 2006;56:477-86.
https://doi.org/10.1099/ijs.0.63980-0
Hong SB, Cho HS, Shin HD, Frisvad JC, Samson RA. Novel Neosartorya species isolated from soil in Korea. Int J Sys Evol Microbiol 2006;56:477-86.
https://doi.org/10.1099/ijs.0.63980-0