During surveys of Eurotiales fungi from soil habitats, a fungal strain, CNUFC U8-51, was isolated. Based on the morphological characteristics and phylogenetic analyses of a combined dataset of four loci (β-tubulin, calmodulin, internal transcribed spacer, and RNA polymerase II second largest subunit), the isolate was identified as Penicillago mirabilis. To our knowledge, this is the first record of the genus Penicillago in Korea, as well as the first record of P. mirabilis in the country. Herein, descriptions, illustrations, and results of the phylogenetic analyses of this species are provided.