Korean Journal of Mycology (Kor. J. Mycol.)
Won-Ho Lee, Young-Jin Park, Ho-Kyung Kim 1,3, Joo-Young Cha 2, Tae Woong Kim 3 and Jae-Mo Sung*
Division of Bio-Resources Engineering,
3Division of Life Science, Kangwon National University, Chuncheon 200-701, Korea
1Mushtech Co. Ltd. Chuncheon 200-161, Korea
2Field Science Center for Northern Bioshpere, Hokkaido University, Sapporo 060, Japan
Correspondence to jmsung@kangwon.ac.kr
Korean Journal of Mycology (Kor J Mycol) December 30, 2005, 33(2):59-63.
https://doi.org/10.4489/kjm.2005.33.2.059
(Received July 13, 2005)
Published: December 30, 2005.
Copyright © 2005 by The Korean Society of Mycology
Altogether twenty isolates were collected from Wakayama, Japan. The optimum mycelial growth of I. obliquus was observed in BMYA and MCM. The optimum temperature and pH for the mycelial growth were 25 oC and 6.0~7.0, respectively. The optimum carbon and nitrogen sources were dextrose and yeast extract, respectively. Similarly, the optimum mineral salt was K2HPO4. The optimum number of mycelial discs for the mycelial growth was 6~7 per 100 ml. Similarly, the optimum culture period was 21~22 days in liquid broth. The optimum brown rice : water ratio was 1 : 1. No difference in mycelial growth was observed in all the four types of tree stumps used.
Carbon sources, Inonotus obliquus, Nitrogen sources, Optimum culture period