Ramaria botrytis is one of the commercially important ectomycorrhizal mushrooms in Korea and the mushroom is only picked in natural forest. This study was conducted to make ectomycorrhizal synthesis of the fungus using Pinus densiflora seedling. Ectomycorrhizal types of the fungus were simple and dichotomous. Diameter of ectomycorrhizal root was 325-380 μm and fungal mantle was subhyaline with 20-30 μm in diameter. On the other hand, optimum temperature for mycelial growth was 25 oC and the fungus grew 5 cm on potato dextrose agar medium in two months.