Byung Sup Kim 1*, Ernest James Rin 2 and Michael D. Coffey 2
1Department of Plant Science, Kangnung-Wonju National University, Gangneung, Korea 210-702
2Department of Plant Pathology and Microbiology, University of California, Riverside, USA
Correspondence to bskim@nukw.ac.kr
Korean Journal of Mycology (Kor J Mycol) June 30, 2009, 37(1):114-116.
https://doi.org/10.4489/kjm.2009.37.1.114
(Received May 27, 2009. Accepted June 10, 2009)
Published: June 30, 2009.
© The Korean Society of Mycology
In this study, we selected suitable broth media for mass production of Phytophthora capsici oospore, investigated oospore germination and secured F1 progeny. Carrot broth and V8C broth were determined most effective for oospore formation by calculating and comparing oospore concentration produced from 8 different liquid media. Eleven strains were selected from P. capsici (CapA)/P. tropicalis (CapB) and 9 crosses were formed. Oospore progeny were produced, isolated and germinated from A1 and A2 combinations of P. capsici (CapA) with P tropicalis (CapB). This resulted in a total number of 129 F1 isolates of P. capsici/P. tropicalis with a 0.64-4.0% (mean 1.85%) oospore germination.
Broth media, F1 progeny, Oospore formation and germination, Phytophthora capsici