Korean Journal of Mycology (Kor. J. Mycol.)
Jong-Kun Kim 1,3, Jin-Hee Kim 1, Won-Sik Kong 2 and Hee-Wan Kang
1Graduate School of Bio.&Information Technology, Hankyong National University, Ansung 456-749, Korea
2Mushroom Research Division, National Institute of Horticultural & Herbal Science, RDA, Suwon 441-707, Korea
3Institute of Genetic engineering, Hankyong National University, Ansung 456-749, Republic of Korea
Correspondence to kanghw2@hknu.ac.kr
Korean Journal of Mycology (Kor J Mycol) June 30, 2010, 38(1):21-24.
https://doi.org/10.4489/kjm.2010.38.1.021
(Received June 22, 2010. Accepted June 23, 2010)
Published: June 30, 2010.
© The Korean Society of Mycology
This study was carried out to select cell wall degrading enzymes for maximizing protoplast yield from Basidiomycetes. The protoplasts were released from spore suspension, mycelia cultured on cellophane membrane, and homogenized mycelia of Flammulina velutipes using commercial cell wall degrading enzymes. The highest yield of protoplasts was obtained from the homogenized mycelia treated with the enzyme combination of Glucanex R 200G and cellulase onozuka R-10. The protocol was also available for Pleurotus ostreatus, P. eryngii, and Hypsizygus marmoreus.
Basidiomycetes, Cell wall degradation enzymes, Protoplast