New shiitake(Lentinula edodes) strain “Sanlim No. 7” was produced by Di-mon hybridization method. Bed-log cultivation of Sanlim No. 7 was performed. Fruit-body production was most at autumn and it is suitable to use as fresh shiitake rather than dried one. The fruit body is hemispherical shape, brown coloured and diameter of pileus is ca. 47 mm. Row of gill is straight, and its width is narrow. Optimum temperature of fruit-body formation was 11~23 oC, and the fruiting is sporadic. The total amount of fruit-body production during 4 years(one generation) was ca. 1.4 kg/log.