Korean Journal of Mycology (Kor. J. Mycol.)
Song Hee Lee 1, Seo-Young Kwak 1, Han Kyu Ko 2 and Hyun-Sook Lee
1Department of Microbiology and Research Institute for Life Science, Gyeongsang National University, Jinju 660-701, Korea
2Forest Mushroom Research Institute, Gyeonggi 469-803, Korea
Correspondence to hslee@gnu.ac.kr
Korean Journal of Mycology (Kor J Mycol) March 31, 2014, 42(1):64-68.
https://doi.org/10.4489/KJM.2014.42.1.64
Received: March 6, 2014; Revised: March 19, 2014; Accepted: March 21, 2014
Published: March 31, 2014.
© The Korean Society of Mycology
This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
Up to date several mycoviruses including Lentinula edodes Spherical Virus (LeSV) have been reported. As fungal virus was spreaded by infested hypae and spores it could be important to use virus-free spawns to eradicate the mushroom virus disease in the culture farm. We tested the imported spawns of Lentinula edodes by PCR whether LeSV was infested them or not. The primer set targeting the RdRp gene of LeSV was prepared based on partial sequence of the LeSV genome. The RT-PCR analysis showed that 87 among 88 imported spawns of L. edodes were infested by LeSV.
Detection, Lentinula edodes, Mycovirus, RT-PCR