Korean Journal of Mycology (Kor. J. Mycol.)
Indexed in SCOPUS, KCI, DOAJ
OPEN ACCESS, PEER REVIEWED
pISSN 0253-651X
eISSN 2383-5249
Research Note

Isolation and Identification of Wild Yeasts from Freshwaters and Soils of Nakdong and Yeongsan River, Korea, with Characterization of Two Unrecorded Yeasts

1Department of Biomedicinal Science and Biotechnology, Paichai University, Daejeon 35345, Korea
2Division of Food Technology, Biotechnology and Agrochemistry, College of Agriculture and Life Sciences, Chonnam National Uni-
versity, Gwangju 61186, Korea

Correspondence to biotech8@pcu.ac.kr

Korean Journal of Mycology (Kor J Mycol) December, 2016, 44(4):350-354.
https://doi.org/10.4489/KJM.2016.44.4.350
Received: September 19, 2016; Revised: November 13, 2016; Accepted: November 21, 2016
Published: December, 2016.
© The Korean Society of Mycology
This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

ABSTRACT

Diverse wild yeast were isolated from freshwaters and soils of Nakdong and Yeongsan rivers in Korea and identified by the comparison of polymerase chain reaction-amplified nucleotide sequences of the internal transcribed spacer region (including the 5.8S rRNA) and D1/D2 regions of 26S rDNA, using BLAST. In total, 15 strains belonging to 9 species were isolated from 25 samples, out of which Aureobasidium pullulans and Cryptococcus bestiolae were dominant. Candida ghanaensis JSF0127 and Meira geulakonigii JSF0130 were identified as unrecorded yeasts, for which their mycological characteristics were investigated. These unrecorded yeasts formed ascospores and grew in yeast extract peptone dextrose medium containing 5% NaCl.

Keywords

Freshwater, Identification,Isolation, Unrecorded yeasts, Wild yeasts

Section