Dong Yeon Suh 1, Seong-Yeol Son 1, Seong Hwan Kim 1*, Sang Tae Seo 2, Kyung Hee Kim 2 and Han Kyu Ko 3
1Department of Microbiology and Institute of Basic Sciences, Dankook University, Cheonan, Chungnam 330-714, Korea.
2Division of Forest Insect Pests and Diseases, Korea Forest Research Institute, Korea Forest Service, Seoul 130-712, Korea
3Forest Mushroom Research Center, National Forestry Cooperative Federation, Yeoju 469-803, Korea
Correspondence to piceae@naver.com
Korean Journal of Mycology (Kor J Mycol) December 31, 2012, 40(4):288-291.
https://doi.org/10.4489/KJM.2012.40.4.288
(Received 15, November 2012., Revised 2, December 2012., Accepted 11, December 2012)
Published: December 31, 2012.
© The Korean Society of Mycology
Korean oak wilt disease caused by Raffaelea quercus-mongolicae is vectored by the ambrosia beetle Platypus koryoensis. To prevent the spread of the disease, the beetle infested oak tree had been cut into logs, covered with plastic vinyl, fumigated with a pesticide, and stored for three years on the site where the tree was cut. This study was carried out to get information on the fungi colonizing the fumigated oak wood. Wood disk samples collected from the fumigated oak logs at two locations in the Taejo Mountain, Cheonan city, were used for fungal isolation. A total of 99 filamentous fungal isolates were obtained from the wood disk samples. Hypocrea spp., Trichoderma spp. and Penicillium spp. were identified based on morphological characteristics and nucleotide sequence analysis of translation elongation factor 1-alpha gene and ITS rDNA region. Trichoderma was the major fungal group. R. quercus-mongolicae, and P. koryoensis were not detected from the fumigated oak wood. Our work provided evidence that after three years of storage, the fumigated oak wilt-diseased logs should be no longer harmful source of oak wilt disease transmission.
Fungal flora, Fumigation, Oak wilt disease, Platypus koryoensis, Raffaelea quercus-mongolicae