Characterization of Monilinia fructicola Associated with Brown Rot of Cherry Fruit in Korea

Hyo-Won Choi1   Sung Kee Hong1   Young Kee Lee1   Young Ju Nam1   Jae Guem Lee1   Hong Sik Shim1   

Abstract

In August 2013, brown rot was observed on cherry fruits (Prunus avium L.) in Hwaseong city, Korea. Fruit rot first appears as small, circular brown spots that increase rapidly in size causing the entire fruit to rot. Grayish spores appear in tufts on rotted areas. Based on these morphological characteristics, the two isolates were identified as Monilinia fructicola. Molecular analysis of 5.8S subunit and flanking internal transcribed spacers (ITS1 and ITS2) was performed to confirm the identification. The ITS sequences had 100% identity with those of other reference M. fructicola isolates of NCBI GenBank. Pathogenicity was tested using spore suspension inoculation on wounded or unwounded cherry fruits. The lesions were observed on wounded and unwounded fruits after inoculation for 7 days. This is the first report on M. fructicola causing brown rot on cherry fruits in Korea.

Acknowledgements

본 논문은 농촌진흥청 국립농업과학원 농업과학기술 연구개발사업(과제번호 : PJ010004)의 지원에 의해 수행되었습니다.

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