Biochemical Characterization of Agaricus bisporus Dikaryon Strains

Hyuk Woo  Kwon1   Jun Young Kim1   Sung Hwan  Min1   Min Ah  Choi1   Youn-Lee  Oh2   Won-Sik  Kong2   Seong Hwan  Kim*1   

Abstract

Button mushroom (Agaricus bisporus) strains from diverse origins were compared in this study to obtain basic information on their growth and biochemical properties that are helpful for breeding. Among 31 dikaryotic strains tested, most strains showed better mycelial growth on oatmeal agar than on MEA and PDA. Mycelia of the mushroom strains revealed β-glucosidase activity the most clearly among the seven extracellular enzymes tested. All the strains showed protease activity, but β-glucosidase activity was found in 27 strains and xylanase activity was found in 30 strains. The activity of avicelase, CM-cellulase, amylase, and pectinase was detected in less than 20 strains. These results implied that enzymatic characteristics could be used as a criterion of strain selection for breeding study.

Acknowledgements

본 연구는 농림축산식품부·해양수산부·농촌진흥청·산림청 Golden Seed 프로젝트 사업(원예종자사업단, 과제번호: 21 3003-04-1-SBJ10)에 의해 수행된 결과로 이에 감사드립니다.

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