Identification of Unrecorded Endophytic Fungi Isolated from Leaves of Woody Plants in Jejudo, Korea

Bong-Hyung  Lee1   Dong-Yeo  Kim1   Hyeok  Park1   Ju-Kyeong  Eo2   Hyang Burm  Lee3   Ahn-Heum  Eom1,*   

1Department of Biology Education, Korea National University of Education
2Bureau of Basic Ecological Research, National Institute of Ecology
3Division of Food Technology, Biotechnology & Agrochemistry, College of Agriculture and Life Sciences, Chonnam National University

Abstract

In this study, endophytic fungi were isolated from the leaves of five species of woody plants in Jeju, Korea, namely Chamaecyparis obtusa, Cryptomeria japonica, Torreya nucifera, Ilex crenata, and Camellia japonica. The isolated fungal endophytes were identified based on their morphological and molecular characteristics including a sequence analysis of the internal transcribed spacer and 26S regions of rDNA and β-tubulin genes. Ten species of fungal endophytes have not been previously reported in Korea, namely Mycosphaerella aleuritidis, Neofusicoccum eucalyptorum, Neofusicoccum parvum, Phyllosticta citrichinensis, Phyllosticta cryptomeriae, Phomopsis cotoneastri, Sphaerulina rhododendricola, Guignardia mangiferae, Lophodermium jiangnanense, and Lophodermium minus.

Acknowledgements

This work was supported by a grant from the National Institute of Biological Resources (NIBR), funded by the Ministry of Environment (MOE) of the Republic of Korea.

Figures & Tables

Fig. 1. Neighbor-joining tree of endophytic fungal strains isolated from leaves of woody plants in Jejudo. Internal transcribed spacer region were used for sequence analysis. was used as an outgroup.