Genotyping of the Wild Mushroom Lentinula edodes from Mt. Jungwang and Mt. Gariwang in Korea

Yeun Sug Jeong¹ Yeongseon Jang¹ Rhim Ryoo¹ Ki-Hwan Kim² Kang-Hyeon Ka^1,*

¹Division of Wood Chemistry and Microbiology, National Institute of Forest Science
²C&K Genomics

Abstract

Lentinula edodes is an edible wild mushroom that can be found in mountainous regions of the Korean peninsula. Wild oak mushrooms were collected from Quercus mongolica at an elevation of more than 1,000 m on Mt. Jungwang and Mt. Gariwang in Gangwon province. We examined 10 oak mushroom strains to evaluate the genetic similarity among strains. Genetic similarity was determined based on the analysis of microsatellite markers (Led A2, Led A8, Led B2, Led B6, and Led D6) registered in the National Center for Biotechnology Information. We also performed dual culture tests on potato dextrose agar for 2 months at 25 °C. The observed heterozygosity across all microsatellites ranged from 0.00 and 0.60 among 5 microsatellite markers, and the polymorphism information content values of Led A2, Led A8, Led B2, Led B6, and Led D6 were 0.0000, 0.8144, 0.6194, 0.4892, and 0.5702, respectively (mean value = 0.4987). Confrontation lines between strains were formed for almost all combinations. In conclusion, the oak mushroom populations of Mt. Jungwang and Mt. Gariwang have mixed gene pools. However, further studies are needed to identify genetic similarities and variations among these populations.

Acknowledgements

This study was supported by grants from the general project (FP 0801-2016-01) of the National Institute of Forest Science and the Golden Seed Project of Breeding of new strains of shiitake for cultivar protection and sub- stitution of import (213003-04-4-SBH10), National Insti- tute of Forest Science, Republic of Korea.

Figures & Tables

Electropherogram of Led A8 microsatellite marker in the wild . Results of genotype analysis performed using the GENEMAPPER v4.0 software on raw data obtained from analysis using a genetic analyzer on NIFoS 1520 (A), NIFoS 1561 (B), NIFoS 2497 (C), NIFoS 2498 (D), and NIFoS 2783 (E). X-axis, DNA size; Y-axis, peak signal height; NIFoS, National Institute of Forest Science.