Identification of Puccinia iridis on Iris domestica in Korea

In-Young Choi1   Young-Joon Choi2   Jin-Young Kim3   Hyeon-Dong Shin4,*   

1Division of Agricultural Environment, Jeollabuk-do Agricultural Research and Extension Services
2Department of Biology, Kunsan National University,
3Division of Horticulture Research, Gyeonggi-do Agricultural Research and Extension Services
4Division of Environmental Science and Ecological Engineering, Korea University

Abstract

A rust fungus on Iris domestica (syn. Belamcanda chinensis) from China was previously identified as Puccinia iridis. Accordingly, the identity of the rust fungus on the same host species in Korea needs to be reexamned. Morphological characteristics of the Korean materials matched with P. iridis. Molecular phylogenetic analyses based on internal transcribed spacer and large subunit rDNA sequences of two representative materials confirmed the identification by high sequence similarities of 100% and 99% with the reference sequences available in NCBI GenBank database. Phylogenetic tree inferred from neighbor-joining method proved them to be clustered in P. iridis group. Thus, the rust fungus on I. domestica in Korea was determined as P. iridis. On the other hand, a previous record that Puccinia belamcandae is another rust agent on I. domestica in Korea should be reconfirmed in future.

Figures & Tables

Fig. 1. on . A, plants heavily infected with ; B, Uredinia formed on the leaf surface; C, Close-up view of erumpent uredinia; D, E, Urediniospores focused on the median view (D) and the surface view (E) observed under light microscope; F, Uredinium showing a group of urediniospores; G, H, Urediniospores observed underscanning electron microscope.