Research Article

Three Novel Endophytic Fungal Species Isolated from Roots of Medicinal Crops in Korea

Hyeok Park1   Chung Ryul Jung2   Ahn-Heum Eom1,*   

1Department of Biology Education, Korea National University of Education
2Special Forest Products Division, National Institute of Forest Science

Abstract

We isolated endophytic fungal strains from the roots of three medicinal crops, Ligusticum chuanxiong, Angelica gigas, and Cnidium officinale, cultivated at Yeongju, Korea. The fungal strains were identified based on their morphological characteristics and molecular analyses of their internal transcribed spacer, large subunit rDNA, and beta-tubulin regions. Thereby, we identified three previously unrecorded endophytic fungal species, Dactylonectria pauciseptata, Rhizopycnis vagum, and Sistotrema sernanderi, in Korea. In this report, we describe the morphological characteristics and phylogenetic analyses of these three fungal species.

Figures & Tables

Fig. 1. Colonies of strain 17E023 (Dactylonectria pauciseptata) grown for 7 days on potato dextrose agar (PDA) (A) and malt extract agar (MEA) (E), macroconidium (D), microconidium (H1) and macroconidium (H2). Colonies of strain 17E011 (Rhizopycnis vagum) grown for 7 days on PDA (B) and MEA (F), conidia (I, J). Colonies of strain 17E012 (Sistotrema sernanderi) grown for 7 days on PDA (C) and MEA (G), basidia (K, L). (scale bars: D=20 μm, H-L=10 μm).