Effect of Perenniporia fraxinea on Eliminating Urushiol from Rhus verniciflua Stokes Stem Bark

Ji-Hyun Lee1   Seok-Tae Jeong1   Ji-Eun Kang1   Han-Seok Choi2,*   

1Department of Agrofood Resources, National Academy of Agricultural Science, RDA, Wanju 55365, Korea
2Department of Agriculture & Fisheries processing, Korea National College of Agriculture and Fisheres, Jeonju 54874, Korea

Abstract

This study evaluated the raw materials for spawning Perenniporia fraxinea, to eliminate urushiol. The growth rates of spawns on grains of millet, brown rice, and wheat were 4.92±0.05, 2.20±0.03, and 1.93±0.03 mm/day, respectively, and the laccase activity was 0.86±0.02, 0.04±0.01, and 0.01±0.00 U/mL, respectively. These observations revealed millet as the most appropriate grain for spawn production in terms of growth rate and enzyme activity. inoculation of lacquer tree (Rhus verniciflua Stokes) stem bark with millet spawns of P. fraxinea resulted in a reduction of its urushiol contents, up to 86.6% on the third day and up to 98.5% on the seventh day. The optimal period of cultivation to eliminate urushiol was three days with 68% of residual flavonoids and 42% of phenolic components. When compared to the product cultivated from liquid spawn, the millet spawn reduced the cultivation period from 10 days to 3 days for eliminating urushiol.

Figures & Tables

Fig. 1. Growth rate of the on spawn media with different grain sources.