Morphological and phylogeny of Plenodomus sinensis and P. collinsoniae, two unreported species isolated from soil in Korea

Than Naing Moe¹ Kallol Das¹ In-Kyu Kang² Seung-Yeol Lee^1,3,* Hee-Young Jung^1,3

¹School of Applied Biosciences, Kyungpook National University, Daegu 41566, Korea
²Department of Horticultural Science, Kyungpook National University, Daegu 41566, Korea
³Institute of Plant Medicine, Kyungpook National University, Daegu 41566, Korea

Abstract

Two unreported fungal isolates, KNU-GW1901 and KNU-AP100C, were collected from soil sample in Gyeongsangbuk-do, Korea. Their cultural and morphological characteristics were examined after 4 weeks of incubation at 25℃ on potato dextrose agar (PDA), malt extract agar (MEA), and oatmeal agar (OA). The conidial shape of KNU-GW1901 was aseptate, hyaline, globose to ellipsoidal, oblong, and reniform to pyriform and 2.61-4.97×1.93-3.61 µm in size, whereas no conidial structures were observed in KNU-AP100C. The internal transcribed spacer (ITS) regions, large subunit (LSU), and small subunit (SSU) sequences were used to determine the taxonomic positions of the strains using the maximum likelihood phylogenetic tree. The isolate KNU-GW1901 was closely clustered with Plenodomus sinensis MFLUCC 17-0767, and KNU-AP100C was closely matched with P. collinsoniae CBS 120227. Based on the findings of morphological, cultural, and phylogenetic analysis, the isolates KNU-GW1901 and KNU-AP100C were identical to the previously described P. sinensis and P. collinsoniae isolates, respectively, which are first reported in Korea.

Figures & Tables

Fig. 1. Cultural and morphological characteristics of sinensis KNU-GW1901. A,colonies on potato dextrose agar; B, colonies on malt extract agar; C, colonies on oatmeal agar; D, pycnidium; E, F, conidia (Scale bars: D-F=10 μm).