Culture Characteristics and Optimal Conditions for Mycelial Growth of Calocybe indica

Gyeong-Jin Min¹ Hea-sung Park¹ Een-ji Lee¹ Chan-Jung Lee^1,*

¹Mushroom Science Division, National Institute of Horticultural and Herbal Science, RDA, Eumseong 27709, Korea

Abstract

Calocybe indica is an edible mushroom commercially cultivated in India and other tropical countries. In this study, the culture characteristics and optimal conditions of milky mushroom strains were determined. The growth temperature and pH range of milky mushrooms was extensively investigated between 15−35 ℃ and pH 3−11. For efficient cultivation, 20 types of nutrient sources were selected that consisted of one of 21 types of carbon sources, 6 organic nitrogen sources, 6 inorganic nitrogen sources, 13 amino acids, 6 organic acids and 12 inorganic salts. The impact of each of the selected nutrition sources and their concentration on growth was investigated. The optimum pH and temperature were determined to be pH 6.0 and 15 ℃, respectively. The optimum concentration of medium elements for the mycelial growth of C. indica was determined to be as follows: carbon source, 2% maltose; organic nitrogen source, 1% yeast extract; inorganic nitrogen source, 0.1% NaNO3; amino acid, 0.7% asparagine; organic acid, 0.07% acetic acid; inorganic salt, 0.07 mM MnSO4.

Figures & Tables

Fig. 1. Phylogenetic relationship of based on intermal transcribed spacer (ITS) rDNA sequences. Number on branch is bootstrap values of 1,000 replicate analysis. Scale bar means the genetic distance between samples.