Neofusicoccum mangiferae and Thyronectria cucurbitula : Unrecorded Endophytic Fungi Isolated from the Leaves of Woody Plants in Korea

Jong-Chul Lee1   Hyeok Park1   Ahn-Heum Eom1,*   

1Department of Biology Education, Korea National University of Education, Cheongju 28173, Korea

Abstract

We isolated endophytic fungi from the leaves of woody plants inhabiting the Ulleungdo Island and Yeosu in South Korea. The fungal strains were identified based on their morphological characteristics in conjunction with phylogenetic analysis, which was performed using sequences of the internal transcribed spacer, large subunit rDNA, and β-tubulin DNA. We confirmed the presence of Neofusicoccum mangiferae and Thyronectria cucurbitula, two endophytic fungal species that had not been previously recorded in Korea. In this report, we describe the morphological characteristics of these fungal strains and results of the DNA-based phylogenetic analysis.

Figures & Tables

Fig. 1.Cultural and morphological characteristics of two endophytic fungal strains. Colonies of 19E448 grown for 7 days on potato dextrose agar (PDA; A, E) and malt extract agar (MEA; B, F), conidia (I, J); Colonies of 19E298 grown for 7 days on PDA (C, G) and MEA (D, H), conidia (K, L) (Scale bars=10 μm).