Isolation and Characterization of Eleven Unrecorded Pezizomycotina Species from Freshwater Ecosystems in Korea

Jaeduk Goh^1,* Yu-Jeong Jeon¹ Hye Yeon Mun¹ Namil Chung¹ Young-Hwan Park¹ Sangkyu Park¹ Hyejin Hwang¹ Wonsu Cheon¹

¹Fungi Research Team, Nakdonggang National Institute of Biological Resources, Sangju 37242, Korea

Abstract

Freshwater fungi are a poly-phylogenetic group of taxonomically diverse organisms. In this study, we isolated diverse fungal strains from various environmental samples obtained from freshwaters in Korea. These strains were identified by performing molecular phylogenetic analyses of rDNA and/or other sequences (beta-tubulin, RNA polymerase II, and translation elongation factor 1). In addition, we examined their morphological characteristics microscopically and cultural characteristics using different media. We identified eleven unrecorded Pezizomycotina species: Cladosporium angulosum , Pseudorobillarda phragmitis, Paraconiothyrium estuarinum, Pseudopithomyces palmicola, Pyrenochaetopsis paucisetosa, Thelebolus globosus, Plagiostoma mejianum, Trichoderma cremeum, Fusarium tanahbumbuense , Coniochaeta endophytica , and Chaetomium tenue . Environmental samples obtained from different freshwater ecosystems in Korea could thus be a good source for isolating and investigating novel fungal species.

Figures & Tables

Fig. 1.Characters of NNIBRFG27319. Mycelial growth on (A) potato dextrose agar (PDA), (B) oatmeal agar (OA), (C) potato carrot agar (PCA), (D) malt extract agar (MEA), (E) corn meal dextrose agar (CMDA), (F)V 8-juice agar (V8A) for 7 days at 25℃. Microscopic observation showed (G) conidia and (H) conidiphore (Scale bars: 10 μm). Phylogenetic tree of NNIBRFG27319 and related species, based on maximum-likelihood analysis of (I) the actin gene sequences. Numbers at the nodes indicate the bootstrap values (>50%) from 1,000 replications. The bar indicates the number of substitutions per position. New isolates from the present study are shown in bold. “T” means type material.