Isolation and Identification of Two Unreported Sordariomycetes Fungi in Korea: Pestalotiopsis clavata and Botryotrichum iranicum

Seong-Keun  Lim1   Leonid  N. Ten1   Diane  Avalos-Ruiz1   Jung-Joo  Ryu1   In-Kyu  Kang2   Seung-Yeol Lee13,*   Hee-Young  Jung13   

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Abstract

Two fungal strains, designated KNUF-21-006 and KNUF-21-028, were isolated from soil samples collected from Gyeongbuk Province, Korea. The strain KNUF-21-006 was similar to other Pestalotiopsis species in terms of morphological characteristics, including whitish to pale brown mycelium, conidial shape, and size. The isolate had aerial hyphae that produced black fruiting bodies on the mycelium. The conidia were fusoid to ellipsoid, four-septate, and appendage-bearing. Phylogenetic analysis using the internal transcribed spacer (ITS) region, translation elongation factor 1-alpha (TEF), and β-tubulin (TUB) gene sequences confirmed that the closest relationship of the isolate at the species level was with Pestalotiopsis clavata . The strain KNUF-21-028 exhibits similar morphological characteristics to other Botryotrichum species, including white aerial mycelium with sulcate and irregular margins, conidial shape, and size. The conidia were globose, single, and hyaline. Upon molecular analysis—using the ITS region, large subunit (LSU) rRNA gene, and TUB gene sequences—the fungus was identified as Botryotrichum iranicum. This is the first record of these fungal species in Korea.

Figures & Tables

Fig. 1.Cultural and morphological characteristics of Pestalotiopsis clavata KNUF-21-006. Cultures were grown at 25℃ for 7 days on potato dextrose agar (PDA) in darkness. A. B. front and reverse view of the colony, C. D. conidia with appendage. Scale bars: C, D=10 μm.