Morphological and Molecular Characterization of the Newly Reported Penicillium pimiteouiense from Field Soil in Korea

Mahesh  Adhikari1   Hyun Seung  Kim1   Hyo Bin Park1   Ki Young Kim1   In Kyu Lee1   Eun Jeong Byeon1   Ji Min Woo1   Hyang Burm Lee2   Youn Su  Lee1,*   

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Abstract

Penicilliumpimiteouiense was discovered in South Korea during an investigation of fungal communities in soil collected from the Gyeongsangbuk-do province. In this study, we performed molecular analysis of this fungal isolate using internal transcribed spacer rDNA, β-tubulin, and Calmodulin gene sequences. We also performed morphological analysis using five agar media, potato dextrose, oatmeal, malt extract, czapek yeast extract, and yeast extract sucrose. In this study, the molecular and morphological analyses of P. pimiteouiense with detailed descriptions and figures has been carried out.

Figures & Tables

Fig. 1.Morphological characteristics of Penicillium pimiteouiense (KNU17-56) grown for 7 days on potato dextrose agar (PDA), czapek yeast extract agar (CYEA), malt extract agar (MEA), yeast extract sucrose agar (YESA), and oatmeal agar (OMA), at 25℃. Front colony from left to right (A-E) and back colony from left to right (F-J) grown on PDA, CYEA, MEA, YESA, and OMA. Conidiophores and conidia images taken by simple microscope (K, L). Scanning electron microscope images of conidiophore (M) and conidia (N) (scale bars: K, M, N=10 μm, L=2 μm).