Six Yeast Strains Isolated from Freshwaters for the First Record in Korea

Yoosun  Oh1   Min Jeong  Seo1   Jaeduk Goh1   Hye Yeon  Mun1,*   

1

Abstract

This study aimed to isolated wild yeasts from freshwater in Korea. The strains were identified by using the D1/D2 domains of the 26S rDNA regions. Consequently, six strains were named as Apiotrichum chiropterorum (NNIBRFG36995), A. domesticum (NNIBRFG32938), A. dulcitum (NNIBRFG33144), A . laibachii (NNIBRFG36991), Saprochaete quercus (NNIBRFG33183) and Tausonia pullulans (NNIBRFG33181). These yeasts have not previously been recorded in Korea, this paper is the first report. We were investigated to the morphological and cultural characteristics of these yeasts. All the strains grew well on Yeast extract peptone dextrose (YPD), Potato dextrose (PD), and Yeast mold (YM) media and in temperature range of 15-30℃. A. domesticum (NNIBRFG32938), A . laibachii (NNIBRFG36991) and T. pullulans (NNIBRFG33181) grew even in 20% glucose containing YPD medium, so they had glucose tolerance. A. domesticum (NNIBRFG32938) and A . laibachii (NNIBRFG36991) had salt resistance as growing even in 5% NaCl containing YPD medium.

Figures & Tables

Fig. 1.Phylogenetic tree of unrecorded yeasts isolated from freshwater and related species based on a Neighbor-joining analysis of the nucleotides sequences of large subunit 26S ribosomal DNA D1/D2 region, using MEGA-X. The sequences of Candida albicans was used as outgroup. The unrecorded yeasts are shown in bold and red.