Acrodontium burrowsianum and Pestalotiopsis humicola: Two Previously Unrecorded Fungal Species Isolated from Conifer Leaves in Korea

Jae-Wook  Choi1   Jung-Min  Lee1   Seok-Yong  Park1   Ahn-Heum  Eom1,*   

1Department of Biology Education, Korea National University of Education, Cheongju 28173, Korea

Abstract

Endophytic fungal strains were isolated from the leaves of two conifer species (Juniperus rigida and Pinus densiflora) in Korea and identified on the basis of their morphological and molecular characteristics. Internal transcribed spacer and large subunit regions of rDNA were used for the phylogenetic analysis, and the translation elongation factor 1-alpha (TEF) and RNA polymerase II second largest subunit (RPB2) genes were analyzed depending on the species. Two fungal species that were previously unrecorded in Korea were identified: Acrodontium burrowsianum and Pestalotiopsis humicola. Their morphological and phylogenetic characteristics are described herein.

Figures & Tables

Fig. 1. Morphological characteristics of Acrodontium burrowsianum KNUE21E086. A, D: Grown for 7 days on potato dextrose agar (PDA) medium. B, E: Grown for 7 days on malt extract agar (MEA) medium. C: Conidia, conidiogenous cells and conidiophore. F: Conidia. Scale bars=10 μm.