Molecular and Morphological Characterization of Three New Fungal Species of the Class Sordariomycetes from Korea

Kallol  Das1   Jae-Ho  Ban1,2   So-Young  Choi1   Seung-Yeol  Lee1,3,*   Hee-Young  Jung1,3   

1College of Agriculture and Life Sciences, Kyungpook National University, Daegu 41566, Korea
2Plant Quarantine Technology Center, Animal and Plant Quarantine Agency, Gimcheon 39660, Korea
3Institute of Plant Medicine, Kyungpook National University, Daegu 41566, Korea

Abstract

Three fungal strains belonging to the class Sordariomycetes were isolated from soil collected on Jeju Island and Gyeongsangbuk-do, Korea. They were identified as Diaporthe endophytica (KNU-JJ-1809), Faurelina indica (KNU-JJ-1830), and Trichoderma ivoriense (KNU-4-KH1). KNU-JJ-1809 produced beta conidia that were straight, curved, hyaline, smooth-walled, with a diameter of 16.5-25.0×0.6-1.7 μm. The conidia of strain KNU-JJ-1830 were hyaline to light green, thin, clavate, round, truncate base, had guttules at both ends, with a diameter of 2.5-5.2×1.7-3.8 μm. The conidia of strain KNU-4-KH1 were oblong or ellipsoidal, smooth-walled, greenish, with a diameter of 2.2-4.4×2.2-3.6 μm. Internal transcribed spacer regions, partial large subunit, translation elongation factor 1-alpha, β-tubulin, and calmodulin genes were used to confirm the strains, and their cultural and morphological characteristics. To our knowledge, this is the first report on D. endophytica, F. indica, and T. ivoriense in Korea.

Figures & Tables

Fig. 1. Cultural and morphological characteristics of KNU-JJ-1809. Colony on potato dextrose agar (A); malt extract agar (B); and oatmeal agar (C). Conidiogenous cells (D-F); beta conidia (G). Scale bars: D-G=10 μm.