Genetic Diversity of Agaricus bisporus Strains by PCR Polymorphism

Kyong-Jin Min12   Jong-Kun kim2   A-Min Kwak1   Won-Sik  Kong2    Oh Youn-Hee3   Hee-Wan Kang*12   


Twelve Universal fungal PCR fingerprint (UFPF) primers that were modified from Universal rice primer (URP) were used to assess genetic diversity of 64 Agaricus strains including 45 A. bisporus strains and other 19 strains of other Agaricus spp. Eight primers, UFPF1, UFPF2, UFPF3, UFPF7, UFPF9, UFPF10, UFPF11, and UFPF12 produced PCR polymorphic bands within and between the Agaricus species. Primer UFPF7 produced specific PCR polymorphic bands that are distinct Korean strain from different strains. Ninety five PCR polymorphic bands were inputted for UPGMA cluster analysis. Forty five strains of A. bisporus are genetically clustered into 8 groups, showing coefficient similarity from 0.75 to 0.9 among them. The varieties, Saea, Saedo, Saejeong and Saeyeon that have recently been developed in Korea were involved in the same group with close genetic relationship of coefficient similarity over 0.96, whereas, other Korean strains were genetically related to A. bisporus strains that were introduced from USA, Eroupe and Chinese.


본 연구는 농림축산식품부, 해양수산부, 농촌진흥청·산림청 Golden Seed 프로젝트 사업(원예종자사업단, 과제번호: 213-003-04-1-SBJ10)에 의해 이루어진 것임.

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