Diversity of Mycotoxigenic Fusarium armeniacum Isolated from Rice Grains at Harvest Time in Korea

Abstract

A total of 509 rice panicle samples were collected at harvest time from fields in 8 provinces from 2010 to 2014. One hundred five grains per sample were plated on potato dextrose agar and 6,658 Fusarium isolates were obtained; among them, 67 were identified as Fusarium armeniacum by sequencing the translation elongation factor 1α (EF-1α) and confirmed by their morphological and cultural characteristics. Considerable variation in conidial size, colony color and EF-1α sequences was observed among the fungal isolates. The ability of 24 F. armeniacum isolates to produce T-2 and HT-2 toxin in potato sucrose agar was determined using liquid chromatography-mass spectrometry. Twenty one isolates produced T-2 and HT-2 toxin, resulting in varying toxin levels among the isolates. The results show that Korean isolates of F. armeniacum have diversity with respect to morphological, cultural, genetic, and toxigenic properties.

Acknowledgements

This study was carried out with the support of “Research Program for Agricultural Science & Technology Development (Project No. PJ008635)”, National Academy of Agricultural Science, Rural Development Administration, Republic of Korea.

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