Optimal Storage Condition of Clubroot Pathogen, Plasmodiophora brassicae for Artificial Inoculation

Abstract

Clubroot, caused by the obligate parasite Plasmodiophora brassicae, is a severe soilborne disease of Brassicaceae. Storage of clubroot gall is important for studies on pathogenicity and race identification. As the current storage method has been used for more than 100 years, a new storage method should be developed and the most efficient way maintaining pathogenicity should be determined. Effects of storage conditions with different storage periods on pathogenicity in galls of kimchi cabbage were examined in a greenhouse. The experiments were performed under six conditions and four temperatures in order to determine the most effective storage conditions for maintenance of pathogenicity. The most effective conditions for clubroot gall storage was the storage of whole gall at -70ºC or storage of filtrate at the same temperature through eight layers of gauze after homogenization of the galls.

Acknowledgements

This work was supported by a grant from the Golden Seed Project (Grant No. 213002043SBI40) of the Ministry of Agriculture, Food and Rural Affairs (MAFRA), Republic of Korea.

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